Journal: PLoS ONE

Article Title: Low-Power Laser Irradiation Suppresses Inflammatory Response of Human Adipose-Derived Stem Cells by Modulating Intracellular Cyclic AMP Level and NF-κB Activity

doi: 10.1371/journal.pone.0054067

Figure Lengend Snippet: hADSCs were pre-treated with or without LPS (50 ng/ml), followed by LPLI (8 J/cm 2 ), and the localization of the NF-κB was determined by fluorescence. The images in the upper panel show the cytoplasmic localization of NF-κB in the control cells. The image in the middle panel shows the nuclear translocation of NF-κB in cells treated with LPS. The image in the lower panel shows that LPLI treatment blocked the nuclear translocation of NF-κB caused by LPS stimulation.

Article Snippet: Cells were co-transfected with the pGL 4.32[luc2P/NF-κB-RE/Hygro] vector (1 µg) (Promega, Madison, WI, USA) and pTK-Renilla vector (20 ng) (Promega, Madison, WI, USA).

Techniques: Fluorescence, Translocation Assay

Journal: PLoS ONE

Article Title: Low-Power Laser Irradiation Suppresses Inflammatory Response of Human Adipose-Derived Stem Cells by Modulating Intracellular Cyclic AMP Level and NF-κB Activity

doi: 10.1371/journal.pone.0054067

Figure Lengend Snippet: ( A ) Intracellular cAMP levels were measured by ELISA in hADSCs treated with LPS, LPLI, SQ22536, and forskolin. ( B ) NF-κB transcriptional activity was assayed 5 hours after LPLI treatment only and combined pretreatment of SQ22536. The data are expressed as the mean ± SD (n = 6). The following statistical levels were applied: *p<0.05 and **p<0.01 compared to the control, #p<0.05 compared to LPS (50 ng/ml), and †p<0.05, ††p<0.01, and †††p<0.001 compared to LPLI (8 J/cm 2 ).

Article Snippet: Cells were co-transfected with the pGL 4.32[luc2P/NF-κB-RE/Hygro] vector (1 µg) (Promega, Madison, WI, USA) and pTK-Renilla vector (20 ng) (Promega, Madison, WI, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay